Rapid Individual Identification by Minisatellite Variant Repeat (MVR)-PCR at D1S8 Locus Using "Exponential Law"

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 参考文献27件
  • SUENAGA Emi
    Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • NAKAMURA Hiroshi
    Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science

この論文をさがす

抄録

We describe an efficient and simple minisatellite variant repeat mapping by PCR (MVR-PCR) method based on the assignment of the tandem array of 29 bp repeating units into a-type, t-type and 0-type (a rarely appearing unamplified unit), from the first repeat unit position (code position 0) of 293 bp in D1S8 locus. After microchip electrophoresis of PCR product amplified from the target DNA of a human hair root, each rung of the ladder at the position of 293 + 29 n bp (n: code position) was detected and the type of repeating unit was determined, i.e., aa, a0, tt, t0, at and 00. The peak area of the rungs from PCR product decreased with increase in code position. We found for the first time that the logarithmic plots of the peak area against the code position showed a linear relationship, which implied that peak areas decrease exponentially. The present method was successfully applied to identify 37 individuals using only a hair root as a biological specimen. This “exponential law” is expected to be an effective tool in forensic science.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 20 (12), 1629-1633, 2004

    社団法人 日本分析化学会

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ