Reversed-phase HPLC Determination of Chlorophyll α′ and Naphthoquinones in Photosystem 1 of Red Algae: Existence of Two Menaquinone-4 Molecules in Photosystem 1 of Cyanidium caldarium
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- YOSHIDA Emi
- Institute of Industrial Science, The University of Tokyo
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- NAKAMURA Akimasa
- Institute of Industrial Science, The University of Tokyo
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- WATANABE Tadashi
- Institute of Industrial Science, The University of Tokyo
書誌事項
- タイトル別名
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- Reversed-phase HPLC Determination of Chlorophyll a' and Naphthoquinones in Photosystem I of Red Algae: Existence of Two Menaquinone-4 Molecules in Photosystem I of Cyanidium caldarium
- Reversed phase HPLC Determination of Chlorophyll アルファ and Naphthoquinones in Photosystem 1 of Red Algae Existence of Two Menaquinone 4 Molecules in Photosystem 1 of Cyanidium caldarium
- Reversed-phase HPLC Determination of Chlorophyll a′ and Naphthoquinones in Photosystem I of Red Algae: Existence of Two Menaquinone-4 Molecules in Photosystem I of Cyanidium caldarium
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説明
Chlorophyll (Chl) a′, the C132-epimer of Chl a, is one of the two Chl molecules constituting the primary electron donor (P700) of photosystem (PS) I of a thermophilic cyanobacterium Synechococcus elongatus. To examine whether PS I of other oxygenic photosynthetic organisms in general contain one Chl a′ molecule in P700, the pigment composition of thylakoid membranes and PS I preparations isolated from red algae Porphyridium purpureum and Cyanidium caldarium was examined by reversed-phase HPLC with particular attention to Chl a′ and phylloquinone (PhQ), the secondary electron acceptor of PS I. The two red algae contained one Chl a′ molecule at the core part of PS I. In PS I of C. caldarium, two menaquinone-4 (MQ-4) molecules were detected in place of PhQ used by higher plants and cyanobacteria. The 1:2:1 stoichiometry among Chl a′, PhQ (MQ-4) and P700 in PS I of the red algae indicates that one Chl a′ molecule universally exists in PS I of oxygenic photosynthetic organisms, and two MQ-4 molecules are associated with PS I of C. caldarium.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 19 (7), 1001-1005, 2003
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679234870784
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- NII論文ID
- 10011245872
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3sXlslGlsL0%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6600197
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- PubMed
- 12880082
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- PubMed
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