Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)<sub>2</sub>PIP(V) as a Nucleic Acid Probe
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- SONG Gongwu
- Analysis and Testing Center Hubei University
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- LI Ling
- Analysis and Testing Center Hubei University
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- LIU Liming
- Analysis and Testing Center Hubei University
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- FANG Guangrong
- Analysis and Testing Center Hubei University
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- LU Shaofang
- College of Chemistry, Wuhan University
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- HE Zhike
- College of Chemistry, Wuhan University
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- ZENG Yun’e
- College of Chemistry, Wuhan University
Bibliographic Information
- Other Title
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- Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe
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Abstract
A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.
Journal
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- Analytical Sciences
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Analytical Sciences 18 (7), 757-759, 2002
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679234932736
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- NII Article ID
- 10011311992
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38XlsVGmsL4%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6220137
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- PubMed
- 12137369
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed