The intercalation of DNA with dopamine by spectroscopic and electrochemical methods
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- LIU Jun
- Department of Chemistry, Tsinghua University Department of Chemistry, Hebei University
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- WANG Zong-Hua
- Department of Chemistry, Tsinghua University
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- LUO Guo-An
- Department of Chemistry, Tsinghua University
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- LI Qing-Wen
- Department of Chemistry, Tsinghua University
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- SUN Han-Wen
- Department of Chemistry, Hebei University
書誌事項
- タイトル別名
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- The Interaction of DNA with Dopamine by Spectroscopic and Electrochemical Methods.
- Interaction of DNA with Dopamine by Spectroscopic and Electrochemical Methods
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説明
It has recently been reported that dopamine may show some biological activities in antitumor and cell apoptosis. We have thoroughly investigated the interaction between dopamine and DNA by CD, UV, fluorescence and electrochemical methods. The results of spectroscopic measurements have indicated that a binding event occurs in a dopamine-DNA system. Besides the electronstatic interaction between a negatively charged DNA molecule and a positively charged dopamine molecule, other binding modes, such as hydrogen-bond and intercalation may also exist in this system. The interaction parameters, including the equilibrium constant and binding numbers, were estimated by an electrochemical method based on the redox current and formal potentials. Both of the two calculation methods showed that the 1:1 type of complex was formed in the dopamine-DNA system and that its equilibrium constant was about 5.85 × 106 M-1. Based on the results of UV, fluorescence and electrochemical experiments in the present study, dopamine may be employed as an effective probe for a DNA assay.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 18 (7), 751-755, 2002
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679234938240
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- NII論文ID
- 10011311965
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38XlsVGms7c%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6220125
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- PubMed
- 12137368
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可