High-Performance Liquid Chromatographic Determination of Oxalic Acid in Tea Using Tris(1, 10-phenanthroline)-ruthenium(II) Chemiluminescence.

WU Fengwu, HE Zhike, LUO Qingyao, ZENG Yun’e

doi:10.2116/analsci.14.971

High-Performance Liquid Chromatographic Determination of Oxalic Acid in Tea Using Tris(1, 10-phenanthroline)-ruthenium(II) Chemiluminescence.

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WU Fengwu

Department of Chemistry, Wuhan University
HE Zhike

Department of Chemistry, Wuhan University
LUO Qingyao

Department of Chemistry, Wuhan University
ZENG Yun’e

Department of Chemistry, Wuhan University

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タイトル別名

High-Performance Liquid Chromatographic

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The chemiluminescence (CL) of Ru(phen)₃²⁺ was applied to HPLC determination of oxalic acid, which was separated by a C₁₈ reverse-phase column with a mobile phase of 0.02 mol/l NH₄Ac, mixed with 0.25 mol/l Ru(phen)₃²⁺ and 2.0 mmol/l Ce(SO₄)₂ (0.08 mol/l H₂SO₄), and then passed through a homemade CL detector. The reaction of Ce(IV) oxidized Ru(phen)₃²⁺ and the oxalic acid emitted light. The detection limit was 6.2×10^-6 mol/l for oxalic acid at a S/N ratio of 3, the relative standard deviation for 5 replicate injections of 1×10^-3 mol/l oxalic acid standard was calculated as 5.6%, and the lin-ear calibration range was 1×10^-5 to 4×10^-3 mol/l. The method was successfully applied to determination of oxalic acid in tea.