Separation and Determination of Fluorescein Isothiocyanate-Labeled Amino Acids by Capillary Electrophoresis with Laser-Induced Fluorescence Detection.
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- TAKIZAWA Keita
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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- NAKAMURA Hiroshi
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
Bibliographic Information
- Other Title
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- Separation and Determination of Fluores
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Description
A method for the separation and determination of amino acids by capillary electrophoresis with laser-induced fluores-cence detection has been developed. At first, the condition for the derivatization of amino acids with fluorescein isothio-cyanate (FITC) was studied; it was proved to be optimal at 50°C for 5 h. Next, the separation of 20 FITC-amino acids was attempted by using an unmodified fused silica capillary. Actually, 18 kinds of FITC-amino acids were separated with 96 mM sodium borate buffer (pH 9.5) containing either 25% acetone (separation system 1) or 10% methanol (separation system 2). The resolution of 19 FITC-amino acids was attained with 80 mM sodium borate buffer (pH 9.2) containing 45 mM α-cyclodextrin (separation system 3). Finally, we succeeded in separating 20 FITC-amino acids by combining two migration systems (2 and 3). The FITC-amino acids were detected by argon-ion laser-induced fluorescence (Ex 488 nm, Em 520 nm). The detection limits of the amino acids obtained with separation systems 1 and 2 were 0.052-1.01 fmol.
Journal
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- Analytical Sciences
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Analytical Sciences 14 (5), 925-928, 1998
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679235038976
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- NII Article ID
- 10002417248
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1cXmslWqurY%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4576989
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed