Galactose-functionalized Magnetic Iron-oxide Nanoparticles for Enrichment and Detection of Ricin Toxin
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- LIU He-Zhu
- Beijing Institute of Pharmacology and Toxicology
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- TANG Ji-Jun
- Beijing Institute of Pharmacology and Toxicology
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- MA Xiao-Xi
- Beijing Institute of Pharmacology and Toxicology
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- GUO Lei
- Beijing Institute of Pharmacology and Toxicology
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- XIE Jian-Wei
- Beijing Institute of Pharmacology and Toxicology
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- WANG Yu-Xia
- Beijing Institute of Pharmacology and Toxicology
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Abstract
To generate a new specific recognition module for the enrichment and detection of ricin, galactose-functionalized silanized magnetic iron-oxide nanoparticles (Gal-SiMNPs) were assembled by amino-silanized MNPs (amino-SiMNPs) and a galactose linker. Amino-SiMNPs were produced by a coprecipitation method, and were coated with double layers of silica and aminosilane by hydrolysis of tetraethoxysilane and 3-aminopropyltriethoxysilane, respectively. A galactose with an amido-acid linker was synthesized by four steps of chemical modification from O-acetyl protected galactose. The diameters of Gal-SiMNPs were characterized as being 60 ± 20 nm. The average amount of galactose-loading and ricin-binding on Gal-SiMNPs was 30 ± 2 μg galactose and 29 ± 2 μg ricin toxin on the surface of 1 mg of Gal-SiMNPs, respectively. Furthermore, a rapid, simple and efficient colorimetric assay was established for the detection of ricin based on the Gal-SiMNPs, and the limits of detection (LODs) of 2 and 4 ng/mL for ricin in physiological buffer and serum were obtained, respectively.
Journal
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- Analytical Sciences
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Analytical Sciences 27 (1), 19-24, 2011
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679235683584
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- NII Article ID
- 10027739443
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10932873
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed