Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献9件 参考文献50件
  • ANAN Yasumi
    Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University
  • HATAKEYAMA Yoshiko
    Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University
  • TOKUMOTO Maki
    Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University
  • OGRA Yasumitsu
    Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University

この論文をさがす

抄録

Two major selenoproteins are present in mammalian serum: extracellular glutathione peroxidase (eGPx) and selenoprotein P (Sel P). The chromatographic behaviors of the two serum selenoproteins were compared in four rodent species, and the selenoproteins in rat serum were identified by measuring enzyme activity and Western blotting. The selenoproteins in rat serum showed a specific chromatographic behavior. In particular, rat eGPx was eluted faster than eGPxs of the other rodent species, although the amino-acid sequences of the rodent species were identical. The elution profiles of Se in rat serum obtained by inductively coupled plasma tandem mass spectrometry (ICP-MS-MS) and ICP-MS were compared. The tandem quadrupoles and the O2 reaction/collision gas completely removed severe interferences with the Se speciation originating from the plasma source and the biological sample matrix. ICP-MS-MS under the O2 mass shift mode gave us more accurate abundance ratios of Se than ICP-MS.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (8), 787-792, 2013

    社団法人 日本分析化学会

被引用文献 (9)*注記

もっと見る

参考文献 (50)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ