Competitive Binding of Abasic Site-Binding Ligands and Masking Ligands to DNA Duplexes for the Analysis of Single-Base Mutation
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- SATO Yusuke
- Department of Chemistry, Graduate School of Science, Tohoku University
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- KAGEYAMA Tomoe
- Department of Chemistry, Graduate School of Science, Tohoku University
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- NISHIZAWA Seiichi
- Department of Chemistry, Graduate School of Science, Tohoku University
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- TERAMAE Norio
- Department of Chemistry, Graduate School of Science, Tohoku University
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抄録
We describe a competitive binding assay for the analysis of single-base mutation by using abasic site (AP site)-specific binding fluorescent ligands and masking ligands bound to the AP site in DNA duplexes. 2-Amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND) can strongly bind to not only cytosine (C), but also thymine (T) opposite an AP site in the DNA duplexes (K11/107 M−1: C, 15; T, 7.0). By contrast, in the presence of lumichrome (Lch) as a masking ligand, ATMND shows a clear binding selectivity for C over T (K11/107 M−1: C, 7.0; T, 0.57), which is ascribed to the effective masking effect of Lch to the ATMND-T interaction in the AP site-containing DNA duplexes. It was shown that the competitive binding of ATMND and Lch to the AP site allowed the highly selective detection of C-related single-base mutation in DNAs amplified by polymerase chain reaction.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 29 (1), 15-19, 2013
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679235804800
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- NII論文ID
- 10031147319
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3s3otFKntg%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 024211992
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- PubMed
- 23303078
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- Web Site
- http://id.ndl.go.jp/bib/024211992
- https://ndlsearch.ndl.go.jp/books/R000000004-I024211992
- https://link.springer.com/content/pdf/10.2116/analsci.29.15.pdf
- https://link.springer.com/article/10.2116/analsci.29.15/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/29/1/29_15/_pdf
- https://search.jamas.or.jp/link/ui/2013248951
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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