Determination of Uric Acid in Plasma by LC-MS/MS and Its Application to an Efficacy Evaluation of Recombinant Urate Oxidase
-
- LUO Xi
- Research Institute of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Central South University
-
- CAI Ningfang
- Research Institute of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Central South University Zhangzhou Municipal Hospital of Fujian Province
-
- CHENG Zeneng
- Research Institute of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Central South University
この論文をさがす
抄録
A liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the determination of uric acid in human plasma was developed and validated. Separation was achieved on a C18 column by the mobile phase of 30% water (containing 0.5% formic acid) and 70% methanol. Quantification was done using a multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 169.1 → m/z 141.1 for uric acid and m/z 171 → m/z 143 for 1,3-15N uric acid (IS) at a positive ionization mode. The calibration curve was established over the range of 0.4096 – 100 mg/L, and the correlation coefficient was better than 0.99. The intra-day and inter-day relative standard deviations were less than 5.1%. The accuracy determined at three concentrations ranged between 92.7 and 102.3%. This method was successfully applied to an efficacy study of intravenous recombinant urate oxidase produced by Escherichia coli in a clinical phase I study.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 29 (7), 709-713, 2013
社団法人 日本分析化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679235853056
-
- NII論文ID
- 10031185232
-
- NII書誌ID
- AA10500785
-
- COI
- 1:STN:280:DC%2BC3sjpvVaktA%3D%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 024671771
-
- PubMed
- 23842413
-
- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I024671771
- https://link.springer.com/content/pdf/10.2116/analsci.29.709.pdf
- https://link.springer.com/article/10.2116/analsci.29.709/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/29/7/29_709/_pdf
- https://search.jamas.or.jp/link/ui/2014028721
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可