Biomolecule Analyses in an Open-Tubular Capillary Chromatography Using Ternary Mixed Carrier Solvents with Chemiluminescence Detection
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- TAKAHASHI Naoya
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- MASUHARA Yuji
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- JINNO Naoya
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- HASHIMOTO Masahiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- TSUKAGOSHI Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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We examined the elution behavior of isoluminol isothiocyanate (ILITC)-labeled biomolecules (α-amino acids, peptides, and proteins) in an open-tubular capillary chromatography system using an untreated fused-silica capillary tube and a water–acetonitrile–ethyl acetate mixture carrier solution. Such an open-tubular capillary chromatography is called “tube radial distribution chromatography (TRDC)” for convenience. A mixture of ILITC and ILITC-labeled biomolecules was analyzed using TRDC with chemiluminescence detection that provided simple instrument without a light source and complex optical devises. The ILITC and the labeled twenty α-amino acids were separated, in this order or the reverse order, or not separated with an organic solvent-rich and water-rich carrier solution. Their elution behavior was considered to be of hydrophilic or hydrophobic nature of ILITC and the labeled α-amino acids. The ILITC and the labeled protein, alcohol dehydrogenase and bovine serum albumin, were separated in this order with an organic solvent-rich carrier solution, while they were eluted in the reverse order with a water-rich carrier solution, based on the TRDC separation performance. The TRDC system worked with the untreated open-tubular capillary tube not using any specific capillary tubes, such as coated, packed, or monolithic.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 28 (4), 351-351, 2012
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679235933696
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- NII論文ID
- 130004827215
- 10030570607
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC38rksFyisw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 023556274
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- PubMed
- 22498461
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- 本文言語コード
- en
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- データソース種別
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- NDL
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- PubMed
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