Mass Transfer in Mesoporous Microparticles Studied by Confocal Fluorescence Recovery after Photobleaching

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  • SATO Tatsumi
    Division of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba
  • HATA Katsuya
    Division of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba
  • NAKATANI Kiyoharu
    Division of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba

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Abstract

The intraparticle diffusion of a fluorescent dye in single microparticles in an aqueous solution was analyzed using fluorescence recovery after photobleaching, with a confocal fluorescence microscope. The fluorescence depth profile of single microparticles, and the fluorescence recovery at the particle center, were measured; further, the intraparticle diffusion coefficient was determined through simulations of three-dimensional diffusion in the respective microparticles. The intraparticle diffusion of coumarin 102 in octadecylsilyl silica gel was limited by the surface diffusion.

Journal

  • Analytical Sciences

    Analytical Sciences 33 (5), 647-650, 2017

    The Japan Society for Analytical Chemistry

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