Motility Measurement of a Mouse Sperm by Atomic Force Microscopy

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  • HSIAO Wesley W.
    Institute of Physics, Academia Sinica Taiwan International Graduate Program—Chemical Biology and Molecular Biophysics, Academia Sinica College of Life Science, National Tsing Hua University
  • LIAO Hsien-Shun
    Institute of Physics, Academia Sinica Department of Mechanical Engineering, National Taiwan University
  • LIN Hsing-Hung
    Taiwan International Graduate Program—Chemical Biology and Molecular Biophysics, Academia Sinica College of Life Science, National Tsing Hua University Genomics Research Center, Academia Sinica
  • DING Ren-Feng
    Institute of Physics, Academia Sinica Department of Mechanical Engineering, National Taiwan University
  • HUANG Kuang-Yuh
    Department of Mechanical Engineering, National Taiwan University
  • CHANG Chia-Seng
    Institute of Physics, Academia Sinica Taiwan International Graduate Program—Chemical Biology and Molecular Biophysics, Academia Sinica

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Abstract

Eukaryotic flagella are responsible for the motile organelles that cause the migration of mammalian sperms. The lashing force and torque of the sperm flagellum contain critical information regarding the sperm health, as important evaluation factors for sperm screening. The objective of the study was to investigate the lashing force and torque of a sperm under physiological conditions using atomic force microscopy (AFM). At a distance of about 18.5 μm from its head-tail junction, a lashing force of 0.96 ± 0.20 nN was measured. Its corresponding lashing torque was 1.77(± 0.37)× 10−14 N·m. The torque increases in proportion to the square of the head-tail junction distance. Our results reasonably conclude that the axonemal motility is linear dependent on the flagellum length of the sperm. Our developed measurement system can consistently determine the lashing force and torque of a sperm, which can contribute to further studies concerning the mechanism of sperm transport and fertilization.

Journal

  • Analytical Sciences

    Analytical Sciences 29 (1), 3-8, 2013

    The Japan Society for Analytical Chemistry

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