Simple and Rapid Immobilization of Coating Polymers on Poly(dimethyl siloxane)-glass Hybrid Microchips by a Vacuum-drying Method
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- KITAGAWA Fumihiko
- Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University
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- NAKAGAWARA Syo
- Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University
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- NUKATSUKA Isoshi
- Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University
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- HORI Yusuke
- Department of Material Chemistry, Graduate School of Engineering, Kyoto University
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- SUEYOSHI Kenji
- Department of Applied Chemistry, Osaka Prefecture University Graduate School of Engineering
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- OTSUKA Koji
- Department of Material Chemistry, Graduate School of Engineering, Kyoto University
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A simple and rapid vacuum-drying modification method was applied to several neutral and charged polymers to obtain coating layers for controlling electroosmotic flow (EOF) and suppressing sample adsorption on poly(dimethyl siloxane) (PDMS)-glass hybrid microchips. In the vacuum-dried poly(vinylpyrrolidone) coating, the electroosmotic mobility (μeo) was suppressed from +2.1 to +0.88 × 10−4 cm2/V·s, and the relative standard deviation (RSD) of μeo was improved from 10.2 to 2.5% relative to the bare microchannel. Among several neutral polymers, poly(vinylalcohol) (PVA) and poly(dimethylacrylamide) coatings gave more suppressed and repeatable EOF with RSDs of less than 2.3%. The vacuum-drying method was also applicable to polyanions and polycations to provide accelerated and inversed EOF, respectively, with acceptable RSDs of less than 4.9%. In the microchip electrophoresis (MCE) analysis of bovine serum albumin (BSA) in the vacuum-dried and thermally-treated PVA coating channel, an almost symmetric peak of BSA was obtained, while in the native microchannel a significantly skewed peak was observed. The results demonstrated that the vacuum-dried polymer coatings were effective to control the EOF, and reduced the surface adsorption of proteins in MCE.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 31 (11), 1171-1175, 2015
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679236161152
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- NII論文ID
- 130005108598
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 026859402
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- PubMed
- 26561262
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- Web Site
- http://id.ndl.go.jp/bib/026859402
- https://ndlsearch.ndl.go.jp/books/R000000004-I026859402
- https://link.springer.com/content/pdf/10.2116/analsci.31.1171.pdf
- https://link.springer.com/article/10.2116/analsci.31.1171/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/31/11/31_1171/_pdf
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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