Paper-based Platform for Urinary Creatinine Detection

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  • SITTIWONG Jarinya
    Department of Chemistry, Faculty of Science, Chulalongkorn University
  • UNOB Fuangfa
    Department of Chemistry, Faculty of Science, Chulalongkorn University

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説明

A new paper platform was developed for the colorimetric detection of creatinine. The filter paper was coated with 3-propylsulfonic acid trimethoxysilane and used as the platform. Creatinine in a cationic form was extracted onto the paper via an ion-exchange mechanism and detected through the Jaffé reaction, resulting in a yellow-orange color complex. The color change on the paper could be observed visually, and the quantitative detection of creatinine was achieved through monitoring the color intensity change. The color intensity of creatinine complexes on the paper platform as a function of the creatinine concentration provided a linear range for creatinine detection in the range of 10 – 60 mg L−1 and a detection limit of 4.2 mg L−1. The accuracy of the proposed paper-based method was comparable to the conventional standard Jaffé method. This paper platform could be applied for simple and rapid detection of creatinine in human urine samples with a low consumption of reagent.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 32 (6), 639-643, 2016

    社団法人 日本分析化学会

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