Detection of Methylated DNA on a Power-Free Microfluidic Chip with Laminar Flow-Assisted Dendritic Amplification
-
- HASEGAWA Kazuki
- Bioengineering Laboratory, RIKEN Department of Materials Science and Technology, Tokyo University of Science
-
- MATSUMOTO Mutsuyoshi
- Department of Materials Science and Technology, Tokyo University of Science
-
- HOSOKAWA Kazuo
- Bioengineering Laboratory, RIKEN
-
- MAEDA Mizuo
- Bioengineering Laboratory, RIKEN
この論文をさがす
抄録
We report on a detection method for methylated DNA on a microfluidic chip, which needs no external power for fluid pumping. The methylated DNA was sandwiched by immobilized probe DNA and an anti-methylcytosine antibody. The fluorescence signal was amplified by our original amplification technology. The detection method was first optimized using a 22-mer DNA sequence, then further validated using a 60-mer DNA sequence adapted from the SEPT9 gene. We were able to detect the methylated 60-mer DNA at 0.4 nM within 18 min.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 32 (6), 603-606, 2016
社団法人 日本分析化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679236548480
-
- NII論文ID
- 130005156499
-
- NII書誌ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 027435472
-
- PubMed
- 27302578
-
- Web Site
- http://id.ndl.go.jp/bib/027435472
- https://ndlsearch.ndl.go.jp/books/R000000004-I027435472
- https://link.springer.com/content/pdf/10.2116/analsci.32.603.pdf
- https://link.springer.com/article/10.2116/analsci.32.603/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/32/6/32_603/_pdf
- https://search.jamas.or.jp/link/ui/2017038158
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可
