Quantitative Determination of Total L-Carnitine in Infant Formula, Follow-up Formula, and Raw Materials by Liquid Chromatography with Tandem Mass Spectrometry
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- NADAOKA Isao
- Department of Infant Milk and Analytical Technology, Research and Development Center, Wakodo Co., Ltd.
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- HATAKEYAMA Emi
- Department of Infant Milk and Analytical Technology, Research and Development Center, Wakodo Co., Ltd.
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- TANADA Chihiro
- Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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- SAKAMOTO Tasuku
- Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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- FUKAYA Shinichi
- Department of Infant Milk and Analytical Technology, Research and Development Center, Wakodo Co., Ltd.
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- AKIBA Takashi
- Department of Infant Milk and Analytical Technology, Research and Development Center, Wakodo Co., Ltd.
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- INOUE Koichi
- Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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- YAMANO Yutaka
- Department of Infant Milk and Analytical Technology, Research and Development Center, Wakodo Co., Ltd.
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- TOYO’OKA Toshimasa
- Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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Abstract
We developed a rapid and useful routine screening assay for total L-carnitine content in various infant formulas and materials by liquid chromatography coupled with a tandem mass spectrometric method (LC-MS/MS) and alkaline hydrolysis. For separation of L-carnitine, a multi-mode octadecylsilane (ODS) column was used that contained ODS ligands, anion ligands, and cation ligands to avoid using ion-pairing agents. The stable isotope L-carnitine-d3 (m/z 165 → 103/85) was used in electrospray MS/MS in the multiple reaction monitoring mode with the ion transitions of m/z 162 → 103/85 for detection and quantitation of L-carnitine. Alkaline hydrolysis of short/medium chain (C2 – C15) acyl-L-carnitines in infant formula was analyzed by LC with quadrupole time-of-flight mass spectrometry (QTOF-MS). The majority of short/medium chain acyl-L-carnitines were hydrolyzed to free L-carnitine. The overall standard deviations for L-carnitine in infant formula, follow-up formula and raw materials ranged from 2.1 to 4.0. The overall mean recoveries ranged from 90.2 to 94.2%.
Journal
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- Analytical Sciences
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Analytical Sciences 30 (8), 839-844, 2014
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679236567552
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- NII Article ID
- 130004677860
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2M%2Fgs1Orug%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 025633305
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- PubMed
- 25109647
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- Web Site
- http://id.ndl.go.jp/bib/025633305
- https://ndlsearch.ndl.go.jp/books/R000000004-I025633305
- https://link.springer.com/content/pdf/10.2116/analsci.30.839.pdf
- https://link.springer.com/article/10.2116/analsci.30.839/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/8/30_839/_pdf
- https://search.jamas.or.jp/link/ui/2015062392
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed