Optical Characterization of the Antigen–Antibody Thin Layer Using the Whispering Gallery Mode

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  • TAJIRI Takeshi
    Industrial Technology Center of Nagasaki Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima
  • MATSUMOTO Shuzo
    Industrial Technology Center of Nagasaki
  • IMATO Toshihiko
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • OKAMOTO Toshihiro
    Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima
  • HARAGUCHI Masanobu
    Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima

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Description

We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen–antibody layer from the shift of the WGM spectra peak.

Journal

  • Analytical Sciences

    Analytical Sciences 30 (8), 799-804, 2014

    The Japan Society for Analytical Chemistry

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