Light Scattering: a Novel Approach to Analyze Protein 4.1R FERM Domain Interaction with Inside-out-vesicles in Solution
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- SHIBA Kohei
- Sysmex Corporation
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- NUNOMURA Wataru
- Center for Geo-Environmental Science, Graduate School of Engineering and Resource Science, Akita University Department of Life Science, Graduate School of Engineering and Resource Science, Akita University
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- TAKAKUWA Yuichi
- Department of Biochemistry, Tokyo Women’s Medical University
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抄録
In this study, we describe a novel application for light scattering, a method widely used for separation of molecules in solution based on their size. We demonstrate that light scattering analysis can monitor the change in particle size of protein 4.1R prior to and after binding to red blood cell inside-out-vesicles in solution. Light scattering constitutes therefore a novel tool to analyze protein-binding association constants.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 28 (6), 613-615, 2012
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679236691712
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- NII論文ID
- 10030758155
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC38jksVKisg%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 023689227
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- PubMed
- 22729049
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可