Light Scattering: a Novel Approach to Analyze Protein 4.1R FERM Domain Interaction with Inside-out-vesicles in Solution

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  • SHIBA Kohei
    Sysmex Corporation
  • NUNOMURA Wataru
    Center for Geo-Environmental Science, Graduate School of Engineering and Resource Science, Akita University Department of Life Science, Graduate School of Engineering and Resource Science, Akita University
  • TAKAKUWA Yuichi
    Department of Biochemistry, Tokyo Women’s Medical University

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In this study, we describe a novel application for light scattering, a method widely used for separation of molecules in solution based on their size. We demonstrate that light scattering analysis can monitor the change in particle size of protein 4.1R prior to and after binding to red blood cell inside-out-vesicles in solution. Light scattering constitutes therefore a novel tool to analyze protein-binding association constants.

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  • Analytical Sciences

    Analytical Sciences 28 (6), 613-615, 2012

    社団法人 日本分析化学会

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