BODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells

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  • TSUCHIYA Akira
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
  • HASHIM Siti N.
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • ISE Shoko
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
  • FURUHATA Takafumi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
  • KAWAI Kiyohiko
    The Institute of Scientific and Industrial Research, Osaka University
  • WAKABAYASHI Rie
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • GOTO Masahiro
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
  • KAMIYA Noriho
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
  • SANDO Shinsuke
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

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Description

An on-cell aptamer sensor has the potential to reveal cell–cell communications by signalling molecules. We attempted to design new fluorescent aptamer sensors for the sensing of IFN-γ and adenine compounds on cells. BODIPY-labeled external quencher-free aptamer sensors have allowed a turn-on detection of the target molecule with improved off/on efficiency.

Journal

  • Analytical Sciences

    Analytical Sciences 32 (5), 543-547, 2016

    The Japan Society for Analytical Chemistry

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