Split Luciferase Complementation for Analysis of Intracellular Signaling
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- HATTORI Mitsuru
- Department of Chemistry, School of Science, The University of Tokyo
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- OZAWA Takeaki
- Department of Chemistry, School of Science, The University of Tokyo
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Abstract
Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein–protein interactions and their related intracellular signaling in living cells. This review presents a summary of recent topics for the analysis of biological events using split luciferase complementation techniques. Different applications such as G-protein-coupled receptor (GPCR) screening, monitoring phosphorylations, protein–protein interactions, and pH differences in living tissues are described.
Journal
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- Analytical Sciences
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Analytical Sciences 30 (5), 539-544, 2014
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679236955648
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- NII Article ID
- 130004442285
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2cjhslelsw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 025440653
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- PubMed
- 24813951
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- Web Site
- http://id.ndl.go.jp/bib/025440653
- https://ndlsearch.ndl.go.jp/books/R000000004-I025440653
- https://link.springer.com/content/pdf/10.2116/analsci.30.539.pdf
- https://link.springer.com/article/10.2116/analsci.30.539/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/5/30_539/_pdf
- https://search.jamas.or.jp/link/ui/2015033225
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed