Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment
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- AKIMARU Michiko
- Faculty of Pharmacy, Keio University
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- OKUBO Kohei
- Faculty of Pharmacy, Keio University
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- HIRUTA Yuki
- Faculty of Pharmacy, Keio University
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- KANAZAWA Hideko
- Faculty of Pharmacy, Keio University
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Abstract
We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogel-modified stationary phase. Aminopropyl silica beads (average diameter, 40 – 64 μm) were coated with poly(N-isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacrylate (BMA) and N,N-dimethylaminopropyl acrylamide (DMAPAAm) were used as the hydrophobic and cationic monomers, respectively, and copolymerized with NIPAAm. To evaluate the use of this SPE cartridge for the analysis of drugs and proteins in biological fluids, we studied the separation of phenytoin and theophylline from human serum albumin (HSA) as a model system. The retention of the analytes in an exclusively aqueous eluent could be modulated by changing the temperature and salt content. These results indicated that this temperature-responsive SPE system can be applied to the pretreatment of biological samples for the measurement of serum drug levels.
Journal
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- Analytical Sciences
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Analytical Sciences 31 (9), 881-886, 2015
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679237000192
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- NII Article ID
- 130005097924
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 026735630
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- PubMed
- 26353953
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- Web Site
- http://id.ndl.go.jp/bib/026735630
- https://ndlsearch.ndl.go.jp/books/R000000004-I026735630
- https://link.springer.com/content/pdf/10.2116/analsci.31.881.pdf
- https://link.springer.com/article/10.2116/analsci.31.881/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/31/9/31_881/_pdf
- https://search.jamas.or.jp/link/ui/2016154595
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed