PNA-tagged Peptide Microarrays for Ratiometric Activity Detection of Cellular Protein Kinases

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  • IKEDA Hiromu
    Department of Applied Chemistry, Kyushu University
  • YAYAMA Yoshihiro
    Department of Applied Chemistry, Kyushu University
  • HATA Akito
    Department of Applied Chemistry, Kyushu University
  • KAMIMOTO Jumpei
    Department of Applied Chemistry, Kyushu University
  • YAMAMOTO Tatsuhiro
    Institute of System, Information Technologies and Nanotechnologies
  • MORI Takeshi
    Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
  • KATAYAMA Yoshiki
    Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University

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Abstract

Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

Journal

  • Analytical Sciences

    Analytical Sciences 30 (6), 631-635, 2014

    The Japan Society for Analytical Chemistry

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