Implantable Patch Sensor for L-Glutamate in Hippocampal Slices

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  • SOMA Natsumi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • WATANABE Narumi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • SHOJI Atsushi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • SUGAWARA Masao
    Department of Chemistry, College of Humanities and Sciences, Nihon University

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抄録

We describe an implantable patch sensor for monitoring L-glutamate in hippocampal slices in submerged and interface preparations. This patch sensor is prepared by excising the cell membrane in the CA3 region of a hippocampal slice in a submerged preparation, and then implanted in the target neuronal region (CA1) of mouse hippocampal slices. The lifetime of the sensor was 3 – 8 min for the interface slice and 40 – 50 min for the submerged slice. The calibration of an implanted sensor can be achieved by adding an L-glutamate solution to a bath (ACSF) solution. The monitoring of L-glutamate release in the CA1 region of mouse hippocampal slices under chemical stimulation with γ-aminobutyric acid (GABA) and potassium chloride (KCl) was demonstrated.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (2), 181-185, 2013

    社団法人 日本分析化学会

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