Gas Chromatographic Analysis of Guanidino Compounds in Sera and Urine of Uremic Patients Using Glyoxal and Ethyl Chloroformate as Derivatizing Reagents
-
- MAJIDANO Subhan Ali
- Institute of Advanced Research Studies in Chemical Sciences, University of Sindh
-
- KHUHAWAR Muhammad Yar
- Institute of Advanced Research Studies in Chemical Sciences, University of Sindh
この論文をさがす
抄録
Gas chromatographic (GC) method has been developed for the determination of the guanidino compounds: guanidine (G), methylguanidine (MG), guanidinoacetic acid (GAA), guanidinopropionic acid (GPA), guanidinobutyric acid (GBA) and guanidinosuccinic acid (GSA) was carried out after precolumn derivatization with glyoxal and ethyl chloroformate from the column HP-5 (30 m × 0.32 mm i.d.) at 90°C for 3 min, followed by a heating rate 25°C/min up to 260°C with a nitrogen flow rate of 2 ml/min. Detection was by FID. The linear calibrations were obtained within 0.1 – 20.0 μmol/L, with limits of detection (LODs) within 0.014 – 0.024 μmol/L. The separation and derivatization was repeatable (n = 6) with relative standard deviations (RSD) within 0.8 – 1.9% in retention time and 0.5 – 1.8% in peak height/peak area. A number of additives and amino acids did not affect the determination. The method was applied for the determination of guanidino compounds from the serum and urine of 9 healthy volunteers and 8 uremic patients and the amounts found were in the range 0.08 – 0.48 and below the limit of detection (LOD) – 345 μmol/L and 1.82 – 13.88 and 0.77 – 432.0 μmol/L with RSDs within 4.2%, respectively.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 29 (2), 221-226, 2013
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679237438336
-
- NII論文ID
- 10031156859
-
- NII書誌ID
- AA10500785
-
- COI
- 1:STN:280:DC%2BC3sznsVehtw%3D%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 024257905
-
- PubMed
- 23400288
-
- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I024257905
- https://link.springer.com/content/pdf/10.2116/analsci.29.221.pdf
- https://link.springer.com/article/10.2116/analsci.29.221/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/29/2/29_221/_pdf
- https://search.jamas.or.jp/link/ui/2013249110
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可