Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

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  • HE Lihong
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Department of Cell Biology, Medical College of Soochow University
  • YANG Li
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
  • XIONG Aizhen
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
  • ZHAO Shujuan
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
  • WANG Zhengtao
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
  • HU Zhibi
    The Moe Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

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抄録

An ultra-performance liquid chromatography/mass spectrometry method to simultaneously quantify vindoline, catharanthine, serpentine and ajmalicine in Catharanthus roseus cell line C20hi is reported. Samples were extracted with 1% acetic acid, basified to pH 10 with ammonia, then extracted with ethyl acetate, dried, reconstituted with methanol–1% acetic acid water solution (1:1, v/v) and analyzed using an acetonitrile–0.1% formic acid gradient as the mobile phase. Detection was carried out by electrospray ionization mass spectrometry in the positive-ion mode with selective ion monitoring. The analysis of one sample was achieved in 6 min. The limits of detection were 0.46 – 0.70 ng/ml in cell samples, and 0.10 – 0.16 ng/ml in medium samples. The linearity of detection was over the wide range of 1.00 – 6250.0 ng/ml. Intra- and inter-day accuracies (recovery 88.0 – 111.8%) and precision (RSD 1.25 – 7.81%) showed the performance of the assay. This method provides a more sensitive and high-throughput technique to quantify the four alkaloids in large amount of samples, and will be helpful in high-production cultivar screening.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 27 (4), 433-, 2011

    社団法人 日本分析化学会

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