Highly selective needle-type glucose sensors prepared by the immobilization of glucose oxidase on γ-polyglutamic acid film

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  • YASUZAWA Mikito
    Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima
  • EDAGAWA Kazuaki
    Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima
  • MATSUNAGA Takuya
    Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima
  • TAKAOKA Hiroki
    Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima Toyo Precision Parts MFG. Co., Ltd.
  • YABUTANI Tomoki
    Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima

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タイトル別名
  • Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on .GAMMA.-Polyglutamic Acid Film
  • Highly selective needle type glucose sensors prepared by the immobilization of glucose oxidase on g polyglutamic acid film

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抄録

Fine needle-type glucose sensors with an outer diameter of less that 0.2 mm were fabricated using a low-cost biodegradable γ-polyglutamic acid (PGA) not only as a glucose oxidase (GOx) immobilizing material, but also as a permselective inner membrane. PGA film was prepared on a cellulose acetate-coated Pt–Ir wire electrode, and GOx was covalently immobilized on its surface using water-soluble carbodiimide. The obtained electrode was practically not affected by the existence of electroactive compounds, and provided long-term stability for approximately one month. It also functioned in horse serum with a good linear relationship between the current and glucose concentrations within the range of 2.8 to 22.4 mM.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 27 (3), 337-, 2011

    社団法人 日本分析化学会

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