A Carbon Dots-based Fluorescence Turn-on Method for DNA Determination
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- BAI WenJun
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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- ZHENG HuZhi
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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- LONG YiJuan
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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- MAO XiaoJiao
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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- GAO Mei
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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- ZHANG Lingyan
- Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
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抄録
In the present work, we found that methylene blue (MB) caused an efficient quenching of the fluorescence of carbon dots (CDots). However, as ct-DNA was added, the fluorescence of CDots was restored reversibly. Under the experimental conditions, CDots were excellent electron donors. MB adsorbed on the surface of CDots and quenched the fluorescence of CDots through an electron-transfer process; and then the addition of DNA caused a restoration of the CDots fluorescence intensity, since DNA could bind with MB and remove it from the CDots. Based on this phenomenon, we developed a new system for DNA detection. A sensitive detection of ct-DNA with a detection limit of 1.0 × 10−6 mol L−1 and a linear detection range from 3.0 × 10−6 to 8.0 × 10−5 mol L−1 with a relative fluorescence intensity of CDots were achieved. To the best of our knowledge, this is the first use of CDots for DNA detection.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 27 (3), 243-, 2011
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679237690368
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- NII論文ID
- 10027906042
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 10997446
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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