Implementation of Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System
-
- KAN Hyo
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
-
- YAMADA Kento
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
-
- SANADA Nobuyuki
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
-
- NAKATA Koyo
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
-
- TSUKAGOSHI Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University Bio-Microfluidic Science Research Center
Search this article
Abstract
Tube radial distribution chromatography based on tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported. The chromatographic system requires specific instruments and treatments for microfluidic flow in the capillary tube. In this study, we have developed a new model of tube radial distribution chromatography, which is comprised of a commercially available HPLC system without any packed separation columns. Separation is performed in an open-tubular pipe (100-μm inner diameter and 350-cm length; temperature, 5°C) connected between the pump and the detector in the HPLC system. An analyte solution is introduced with a sample injector (2-μL volume) and a ternary water/acetonitrile/ethyl acetate mixed solution (volume ratio of 3:8:2) is delivered as an eluent solution into the pipe at a flow rate of 10-μL min−1. Fused silica and stainless pipes can separate 1-naphthol and 2,6-naphthalenedisulfonic acid, but a polyetheretherketone pipe cannot. The obtained data provides an important clue to practical developments in separation science.
Journal
-
- Analytical Sciences
-
Analytical Sciences 34 (2), 239-241, 2018
The Japan Society for Analytical Chemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679237972224
-
- NII Article ID
- 130006342046
-
- NII Book ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL BIB ID
- 028818226
-
- PubMed
- 29434113
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed