An Improved Protocol for Better Detection of Protein Using 8-Anilino-1-naphthalenesulfonate

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  • MA Weide
    Laboratory of Gynecology and Obstetrics, People’s Hospital of Wenzhou Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College
  • ZHU Guanghui
    The Second Affiliated Hospital of Wenzhou Medical College
  • ZHAO Ting
    Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College
  • CONG Weitao
    Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College
  • YE Weijian
    Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College
  • JIN Litai
    Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College

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抄録

This study describes the optimum conditions at the staining time and the signal intensity for using 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescent probe to detect proteins in SDS-PAGE. Using the optimized protocol, protein can be easily detected by short time fixing (20 min) and washing (2 × 5 min), followed by 10 min of staining. As low as 1 – 2 ng of the protein band can be detected, approximately thirty-fold higher than that of the original protocol. Furthermore, the compatibility of the staining method with MS was also explored by comparing the peptide mass fingerprinting results data of serial dilutions of BSA and ovalbumin stained by ANS with SYPRO Ruby.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (2), 255-262, 2013

    社団法人 日本分析化学会

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