Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension
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- SATO Kae
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- NAKAJIMA Minori
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- TOKUDA Sana
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- OGAWA Aiko
- Division of Molecular and Cellular Medicine, Department of Clinical Science, National Hospital Organization Okayama Medical Center
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抄録
There is an urgent need to develop novel in-vitro models to mimic the disease conditions in pulmonary hypertension (PH). We developed a microfluidic cell culture device for PH studies that withstood high shear stress. Techniques were also developed for cell recovery from the microchannel and mRNA isolation from the collected cells. Using this device, we found that shear stress caused a 7.5-fold increase in the transcription levels of a PH-related molecule, Cyclin D1.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 32 (11), 1217-1221, 2016
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238173568
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- NII論文ID
- 130005277935
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 027714060
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- PubMed
- 27829629
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- Web Site
- http://id.ndl.go.jp/bib/027714060
- https://ndlsearch.ndl.go.jp/books/R000000004-I027714060
- https://link.springer.com/content/pdf/10.2116/analsci.32.1217.pdf
- https://link.springer.com/article/10.2116/analsci.32.1217/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/32/11/32_1217/_pdf
- https://search.jamas.or.jp/link/ui/2017176362
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
