Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry
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- HIYAMA Eiso
- Natural Science Center for Basic Research and Development, Hiroshima University
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- ALI Ahmed
- Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN
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- AMER Sara
- Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN
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- HARADA Takanori
- Natural Science Center for Basic Research and Development, Hiroshima University
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- SHIMAMOTO Kazumi
- Natural Science Center for Basic Research and Development, Hiroshima University
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- FURUSHIMA Rie
- Quantitative Biology Center (QBiC), RIKEN
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- ABOULEILA Yasmine
- Faculty of Pharmacy, Misr International University Quantitative Biology Center (QBiC), RIKEN
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- EMARA Samy
- Faculty of Pharmacy, Misr International University
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- MASUJIMA Tsutomu
- Quantitative Biology Center (QBiC), RIKEN
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Direct trapping of a single floating cell, i.e. a white blood cell from a drop of blood, within a nanospray tip was followed by super-sonication after the addition of ionization solvent. Molecular detection of an increased number of peaks with a higher intensity and a wider m/z range, which extends from metabolites to lipids, was acquired than of that without sonication. This method was applied to a few separated circulating tumor cells (CTC) from a neuroblastoma patient’s blood to obtain their lipido-metabolomic molecular profile at the single cell level. In addition to vital molecules such as amino acids, catechol amine metabolites, which are specific to neuroblastoma, and drugs included in the patient’s course of therapy were detected. This established “direct single-cell lipido-metabolomic method” seems to be useful for direct and wide range molecular detection not only for many live single-cells, but also for rare cells, such as CTCs, for future molecular diagnosis.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 31 (12), 1215-1217, 2015
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238184832
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- NII論文ID
- 130005129718
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 027106620
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- PubMed
- 26656808
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- Web Site
- http://id.ndl.go.jp/bib/027106620
- https://ndlsearch.ndl.go.jp/books/R000000004-I027106620
- https://link.springer.com/content/pdf/10.2116/analsci.31.1215.pdf
- https://link.springer.com/article/10.2116/analsci.31.1215/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/31/12/31_1215/_pdf
- https://search.jamas.or.jp/link/ui/2016219463
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
