Application of a Novel Near Infrared-Fluorescence Giant Vesicle- and Polymerasome-based Tissue Marker for Endoscopic and Laparoscopic Navigation

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  • HATAYAMA Hirosuke
    Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University
  • TOYOTA Taro
    Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University Department of Bioinformatics, Graduate School of Medicine, Chiba University Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo
  • HAYASHI Hideki
    Center for Frontier Medical Engineering, Chiba University Department of Frontier Surgery, Graduate School of Medicine, Chiba University
  • NOMOTO Tomonori
    Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University
  • FUJINAMI Masanori
    Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University

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Abstract

In this study, we describe the development of a novel tissue marker that can be injected from within the digestive tract by using an endoscopic instrument, and visualized using near-infrared (NIR) fluorescence imaging. The marker was prepared in three steps, (i) mixing NIR-fluorescent indocyanine green (ICG) with giant vesicles (GVs) of lecithin, (ii) suspending the ICG-containing giant vesicles (ICG-GV) in an oil phase dissolving polyglycerol-polyricinoleate (PGPR), and (iii) centrifugation of the suspension layered on a buffered solution to obtain a giant polymer vesicle (polymerasome) containing ICG-GV. We injected the tissue marker into the inner gastric surface of an anesthetized pig using an endoscopic syringe, and observed the injection site using a fluorescence laparoscopic camera. The diameter of the spot blur was approximately 2 cm over a 5-h period, demonstrating the utility of this procedure as a tissue marker for tumor marking, and suggesting its potential for assisting navigation during surgical procedures.

Journal

  • Analytical Sciences

    Analytical Sciences 30 (2), 225-230, 2014

    The Japan Society for Analytical Chemistry

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