Simple and Rapid Separation of Soyasaponin Bb from a Soy Extract
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- KATANO Hajime
- Department of Bioscience, Fukui Prefectural University
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- OKAMOTO Nobuhiro
- Department of Bioscience, Fukui Prefectural University
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- TAKAKUWA Masahiro
- Department of Bioscience, Fukui Prefectural University
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- TAIRA Shu
- Department of Bioscience, Fukui Prefectural University
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- KAMBE Taiho
- Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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- TAKAHASHI Masakazu
- Department of Bioscience, Fukui Prefectural University
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説明
A simple method to separate soyasaponin Bb from a soy extract is presented. This method is based on the difference in the solubility of soyasaponin Bb and Ba and other components into 3:7 and 1:1 (v/v) acetone–water mixed solvents. The crude soyasaponin consisting of soyasaponins Aa, Ab, Ba, and Bb at the 10 wt% level and other components was examined as the soy extract. A 10 mg quantity of the crude soyasaponin was mixed with 1 mL of the 3:7 acetone–water containing 0.1 mol/L HCl, and the supernatant was removed to obtain a precipitate, which was found to contain mainly soyasaponins Bb and Ba. The precipitate was mixed with 0.4 mL of the 1:1 acetone–water containing 0.1 mol/L HCl; the supernatant was transferred, and was mixed with 0.6 mL of water to obtain a precipitate, which was found to contain mainly soyasaponin Bb. The yield was ca. 30%, which may be much higher than that by the conventional preparative chromatographic approach. The separation method is rapid and easy to carry out, and is useful for the preparation of a soyasaponin Bb sample.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 31 (2), 85-89, 2015
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238211328
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- NII論文ID
- 130004827460
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 026098233
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- PubMed
- 25746805
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可