Simple, Colorimetric Detection of MicroRNA Based on Target Amplification and DNAzyme
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- YAN Chunyan
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
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- JIANG Cheng
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
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- JIANG Jianhui
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
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- YU Ruqin
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
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説明
Considering the crucial role played by microRNAs (miRNAs) in biological processes, we developed a novel strategy for simple and colorimetric detection of miRNA by combining target amplification with DNAzyme. Throughout the work, a 22-nt oligonucleotide sequence was used as a model analyte. A label-free hairpin probe (HP) was used as a simple platform for sensing the target. In the presence of the target, the HP was opened, and then the isothermal circular strand-displacement process occurred with the help of a primer, deoxynucleotide solution mixture (dNTPs), Klenow fragment exo− polymerase, and Nb.BbvCI nicking enzyme. As a result, the target was recycled and multicopies of target analogues were generated that function in the same manner as the target, accompanied by the accumulation of signal elements. In this work, as low as 0.5 fM nucleic acid target was detected by horseradish peroxidase-mimicking DNAzyme catalyzing the oxidation of ABTS2− to colored ABTS•−.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 29 (6), 605-610, 2013
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238615424
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- NII論文ID
- 10031177782
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3sjgsF2msw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 024586158
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- PubMed
- 23749125
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可