Analytical Methods for the Detection and Purification of ε-Poly-L-lysine for Studying Biopolymer Synthetases, and Bioelectroanalysis Methods for Its Functional Evaluation
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- KATANO Hajime
- Department of Bioscience, Fukui Prefectural University
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- UEMATSU Kohei
- Department of Bioscience, Fukui Prefectural University
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- MARUYAMA Chitose
- Department of Bioscience, Fukui Prefectural University
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- HAMANO Yoshimitsu
- Department of Bioscience, Fukui Prefectural University
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This article describes new analytical methods for studying biopolymer ε-poly-L-lysine (εPL). The produced amount of εPL in culture broth can be determined based on the precipitation of polycationic εPL with a colored heteropolymolybdate anion and the color change of the supernatant. The product can be separated and purified by precipitation with the tetraphenylborate anion and reprecipitation in the form of the hydrochloride salt. These methods have been applied advantageously to the screening of εPL-synthetase. Also, pyrophosphate can be determined colorimetrically based on the formation of 18-molybdopyrophosphate species. The pyrophosphate determination has been successfully applied to the assay of adenylation enzyme, which plays important roles in the biosynthetic mechanism. Under certain conditions, εPL associates with a redox enzyme, glucose oxidase. The effect of the adduction on the stability and reaction rate of the enzyme can be evaluated by measuring the bioelectrocatalytic current, which is related to the enzyme activity. Electrochemical studies showed new applications of εPL as an enzyme stabilizer and a reaction enhancer.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 30 (1), 17-24, 2014
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238652928
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- NII論文ID
- 130004442239
- 40019949573
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2czkvF2hsw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 025160221
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- PubMed
- 24420240
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可