薄層クロマトグラフィーによるAldosteroneの分離について

DOI Web Site PubMed

書誌事項

タイトル別名
  • Separation of aldosterone, 18-hydroxycorticosterone and corticosterone by thin layer chromatography
  • ハクソウ クロマトグラフィー ニ ヨル Aldosterone ノ ブンリ ニ ツイテ

この論文をさがす

説明

The corticoid was extracted with chloroform from the rat adrenals and this solution was then passed through a small column of florisil. The eluates were evaporated to dryness, then the dry residue was dissolved in chloroform and was deposited on to the plates which was coated with silica gel. The solvent systems were chloroform-aceton (3: 2), and ascending chromatography was carried out. The Rf values of aldosterone, 18-hydroxycorticosterone and corticosterone in the systems were respectively 0.33, 0.18 and 0.56, and over-all recovery throughout the separation steps was 75 per cent. These corticoids have been thus separated by thin layer chromatography and the method is available for the study of adrenal activity, especially in the rat.

収録刊行物

  • 日本薬理学雑誌

    日本薬理学雑誌 66 (6), 651-658, 1970

    公益社団法人 日本薬理学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ