家畜けい動脈の抗原性除去に関する免疫薬理学的研究

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タイトル別名
  • Studies on the antigenicity of swine arterio graft
  • カチク ケイドウミャク ノ コウゲンセイ ジョキョ ニ カンスル メンエキ ヤ

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抄録

To obtain better arterio grafts, we prepared three samples from adventitia of swine carotid arteries, and their antigenicity was studied with immunological procedures. Sample I was prepared from swine carotid arteries by eliminating the extraneous fat and connective tissues. Sample II was prepared from Sample I by treatment with chymotrypsin, and Sample III was prepared from Sample II by treatment with 3% glutaraldehyde for 14 days. From the three samples, the water soluble protein fractions were extracted, and abbreviated as E-I, E-II and E-III, respectively. Immunopharmacological tests in guinea pigs and rabbits including active and passive anaphylaxis, passive cutaneous anaphylaxis, Schultz-Dale test and Arthus's phenomenon among these extracts revealed cross reactions between E-I and E-II. Also, precipitin test and Ouchterlony's method, precipitation was observed between E-I and E-II, and it was suggested that there might be at least a common antigen between E-I and E-II. The rabbits which had been implanted subcutaneously at the back with the minced Sample I evoked severe reactions and died after intravenous injection of E-I at the 3rd or 5th month after implantation. No reaction was observed between Sample III and E-III in the same experiment. From the above results, it was suggested that the antigenicity of swine carotid arteries could be eliminated by the treatment with chymotrypsin and glutaraldehyde.

収録刊行物

  • 日本薬理学雑誌

    日本薬理学雑誌 73 (8), 919-929, 1977

    公益社団法人 日本薬理学会

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