Establishment of tea cell lines with high growth rate

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  • 増殖能の高いチャ細胞培養系の確立
  • ゾウショクノウ ノ タカイ チャ サイボウ バイヨウケイ ノ カクリツ

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Calluses originated from 8 cultivars and 4 strains of tea (Camellia sinensis L.) and tumor tissues of cv. "Yabukita" induced by Agrobacterium tumefaciens or A. rhizogenes were used to evaluate the effects of hormonal levels, illumination, and subculture intervals on growth rate. Growth of Agrobacterium induced tissues required neither naphthaleneacetic acid (NAA) nor benzyladenine (BA), whereas growth of "Yabukita" callus reached its maximum on B5 agar medium containing 1-10μM NAA and 1μM BA. By selecting fragile portion of callus tissue, "Yabukita"."Chinshindapan" and PKS47 calluses became highly proliferative. These cell lines showed more vigorous growth in B5 liquid medium than on the solid medium. Illumination reduced the growth of these cell lines. Highest growth rate of "Yabukita" cells was obtained when the cells were subcultured in liquid medium every 5th day. At the best condition, doubling time on fresh weight basis was 2.9. 2.3 and 4.0 days for "Yabukita". "Chinshindapan" and PKS47 cells, respectively. Such high rate of growth had not been reported to date for tea cells.

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