オキシフルオルフェン抵抗性ダイズ細胞のプロトポルフィリノーゲン酸化酵素阻害除草剤に対する交差抵抗性

臼井 健二, ポーンプロム トッサポン, 松本 宏, 白倉 伸一, 石塚 皓造

doi:10.3719/weed.40.187

Characterization of cross-tolerance of selected oxyfluorfen-tolerant and non-selected (normal) soybean cell lines to protoporphyrinogen oxidase (Protox) inhibiting herbicides (oxyfluorfen, 2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl) benzene; bifenox, methyl 5-(2, 4-dichloro-phenoxy)-2-nitrobenzoate; nitrofen, 2, 4-dichloro-1-(4-nitrophenoxy) benzene; and oxadiazon, 3-[2, 4-dichloro-5-(1-methylethoxy) phenyl]-5-(1, 1-dimethylethyl)-1, 3, 4-oxadiazol-2-(3H)-one) or acetolactate synthase (ALS) inhibiting herbicide (bensulfuron-methyl, methyl α-[[3-(4, 6-dimethoxypyrimidin-2-yl) ureido] sulfonyl]-o-toluate, BSM) was determined. The sensitivities of both cell types to oxyfluorfen were compared by determination of the growth rates and target enzyme inhibition using various Protox inhibiting herbicides. On the I₅₀ values of growth, the tolerant cells showed about 100-, 200-, 5, 000-, and >30, 000-fold more tolerance than the normal cells to oxyfluorfen, oxadiazon, bifenox, and nitrofen, respectively. The cells were found to have cross-tolerance to all Protox inhibiting herbicides tested, however, a lack of cross-tolerance (<1.0) to BSM was observed. Determination of the inhibition on Protox activity showed that the sensitivity of the enzyme preparations between the two cell types differed about 15-fold to oxyfluorfen, 30-fold to oxadiazon, 45-fold to bifenox, and 100-fold to nitrofen. There was a positive correlation between the tolerance ratio determined by growth rate and that at the enzyme level.

オキシフルオルフェン抵抗性ダイズ細胞のプロトポルフィリノーゲン酸化酵素阻害除草剤に対する交差抵抗性

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オキシフルオルフェン抵抗性ダイズ細胞のプロトポルフィリノーゲン酸化酵素阻害除草剤に対する交差抵抗性

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