リョクトウ(Vigna radiata (L.) Wilczek) 子葉培養のカルス誘導と植物体再分化におよぼす種子の前処理と異なった光強度の影響
書誌事項
- タイトル別名
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- Effect of Seed Preconditioning and Culture under Different Light Intensities on Callus Induction and Regeneration in Excised Cotyledon Cultures of Mungbean(Vigna radiata(L.) Wilczed).
- Effect of Seed Preconditioning and Culture under Different Light Intensities on Callus Induction and Regeneration in Excised Cotyledon Cultures of Mungbean(Vigna radiata(L.)Wilczek)
- Effect of Seed Preconditioning and Cult
この論文をさがす
抄録
Different seed preconditioning treatments and light intensities were tested for their effect on the callus induction and regeneration in cotyledon cultures of mungbean ( Vigna radiata (L.) Wilczek). There were six preconditioning treatments evaluated including imbibition in sterile distilled water, inoculation in plain agar, and inoculation in 6-benzyladenine (6-BA) supplemented media over a varing period of time. The cultures were incubated in two light intensity conditions; namely, full light (50 μmol m-2 sec-1) and minimum light (8.5 μmol m-2 sec-1). Results of the study showed that (1) plain imbibition of seeds in sterile distilled water for 16 hours was more favorable for regeneration than preconditioning with 6-BA enriched media, and (2) preconditioning of callus formation and better callus growth. Other important observation. was the differential response to plantlet regeneration under different regeneration media. Plantlet regeneration in B5 basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA was relatively higher than the other regeneration media under full light intensity while Murashigc and Skoog (MS) basal medium and MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA had highter plantlet regeneration under minimum light intensity.
収録刊行物
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- 日本作物学会紀事
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日本作物学会紀事 66 (1), 67-75, 1997
日本作物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679258386432
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- NII論文ID
- 110001735028
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- NII書誌ID
- AN00189888
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- ISSN
- 13490990
- 00111848
- http://id.crossref.org/issn/0011183X
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- NDL書誌ID
- 4161358
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可