The Mechanism of Maitotoxin-Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes.

Satoh Eiki, Ishii Toshiaki, Nishimura Masakazu

doi:10.1254/jjp.85.98

The Mechanism of Maitotoxin-Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes.

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Satoh Eiki

Department of Pharmacology, University of Obihiro School of Veterinary Medicine
Ishii Toshiaki

Department of Pharmacology, University of Obihiro School of Veterinary Medicine
Nishimura Masakazu

Department of Pharmacology, University of Obihiro School of Veterinary Medicine

書誌事項

タイトル別名

Mechanism of Maitotoxin Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes

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説明

The present study was conducted to elucidate the mechanism of the maitotoxin (MTX)-induced increase in intrasynaptosomal free calcium level ([Ca²⁺]_i). The MTX (1 ng/ml)-induced increase in [Ca²⁺]_i was partially inhibited by the omission of extracellular Ca²⁺ (Ca²⁺_e) or the addition of verapamil, but not by adding nifedipine, ω-agatoxin IVA, ω-conotoxin GVIA and ω-conotoxin MVIIC. An increase in [Ca²⁺]_i in the absence of Ca²⁺_e was sensitive to procaine, TMB-8, genistein and verapamil, but not to ryanodine and U-73122. These results may suggest that MTX increases [Ca²⁺]_i by stimulating Ca²⁺ entry through voltage-independent nonselective cation channels and Ca²⁺ release from stores through a phospholipase C-γ1-mediated pathway in rat cerebrocortical synaptosomes.