ミシマサイコ種子の発芽に関する研究 : 第6報 ミシマサイコ種子の発芽抑制物質(その2)

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  • Studies on the Germination of Seeds of Bupleurum falcatum. L. : VI. The germination inhibitors of Bupleurum falcatum seeds(2)
  • ミシマサイコ種子の発芽に関する研究-6-ミシマサイコ種子中の発芽抑制物質-2-
  • ミシマサイコ シュシ ノ ハツガ ニカンスルケンキュウ 6 ミシマサイコ シュ

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In the previous article, two substances named inhibitors A and B were isolated from the seed coats of Bupleurum falcatum seeds. In present experiments, these inhibitors were developed on thin-layer chromatograms and chemical and physiological properties of inhibitors were observed. The results obtained are as follows: 1. On the thin-layer chromatograms using the solvent of n-butyl alcohol saturated with ammonia, eight spots were found from inhibitor A, while nine spots were found from inhibitor B. These results were the almost same with those of separation by paper chromatography. The separating power of thinlayer chromatography, however, were better than that of paper chromatography. The inhibition of germination in lettuce seeds was observed with only one spot of inhibitor B, which showed the typical color reaction with the phenol reagents. 2. Under the UV light of spectrophotometer, both inhibitors A and B showed the maximum absorption at 280 nm, though different absorption spectra were observed between inhibitor A and B. 3. When inhibitor B, which showed remarkable physiological activities, was applied to non-dormant Bupleurum falcatum seeds with soaking in water solution at various concentration, it was observed that RNA content in seeds was not affected but amylase activity was lowered at the concentration of more than 50 ppm.

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