Spinorphin, an Endogenous Inhibitor of Enkephalin-Degrading Enzymes, Potentiates Leu-Enkephalin-Induced Anti-allodynic and Antinociceptive Effects in Mice.
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- Honda Motoko
- Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo Laboratory of CNS Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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- Okutsu Hiroko
- Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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- Matsuura Tomoyuki
- Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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- Miyagi Tomohiro
- Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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- Yamamoto Yukio
- Department of Molecular Oncology, The Tokyo Metropolitan Institute of Medical Science
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- Hazato Tadahiro
- Department of Molecular Oncology, The Tokyo Metropolitan Institute of Medical Science
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- Ono Hideki
- Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo Laboratory of CNS Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University
Bibliographic Information
- Other Title
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- Spinorphin, an endogeneous inhibitor of enkephalin-degrading enzymes, protentiates leu-enkephalin-induced anti-allodynic and antinociceptive effects in mice
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Description
Spinorphin (LVVYPWT) has been isolated from the bovine spinal cord as an endogenous inhibitor of enkephalin-degrading enzymes. It has been reported that spinorphin has an antinociceptive effect, inhibitory effect on contraction of smooth muscle and anti-inflammatory effect. In the present study, the effects of leu-enkephalin and spinorphin on allodynia and mechanical and thermal nociceptions were examined in vivo using mice. Intrathecal (i.t.) administration of leu-enkephalin or spinorphin inhibited the allodynia induced by intrathecal nociceptin in a dose-dependent manner. Furthermore, spinorphin enhanced the inhibitory effect of enkephalin on allodynia induced by nociceptin. Naloxone antagonized both inhibitory effects of leu-enkephalin and spinorphin, suggesting that the endogenous opioidergic system can modulate allodynia. Intracerebroventricular (i.c.v.) administration of leu-enkephalin increased the nociceptive threshold of heat or mechanical stimulation to a mouse. Although i.c.v. administration of spinorphin had no effect on the threshold of heat or mechanical stimulation, spinorphin enhanced and prolonged the antinociceptive effect of leu-enkephalin. The enhancement of spinorphin on the antinociception produced by leu-enkephalin was reversed by pretreatment with naloxone. From these results, it is suggested that the effects of spinorphin on enkephalin-induced anti-allodynic and antinociceptive effects are due to inhibition of enkephalin-degrading enzymes.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 87 (4), 261-267, 2001
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390282679263150080
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- NII Article ID
- 10007944062
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DC%2BD3MXptlKkuro%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 6019124
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- PubMed
- 11829145
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- Web Site
- http://id.ndl.go.jp/bib/6019124
- https://ndlsearch.ndl.go.jp/books/R000000004-I6019124
- https://api.elsevier.com/content/article/PII:S0021519819302434?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819302434?httpAccept=text/plain
- https://search.jamas.or.jp/link/ui/2002119854
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed