A Single Stranded DNA-Binding Protein, ssCRE-BP/Pur.ALPHA., in Rat Lung and Its Increase in Allergic Airway Inflammation.

DOI Web Site Web Site Web Site Web Site View 1 Remaining Hide 39 References
  • Wei Er-Qing
    Present address: Department of Pharmacology, Zhejiang Medical University
  • Irie Yasuyuki
    Department of Pharmacology, Osaka University Medical School
  • Kuo Che-Hui
    Department of Pharmacology, Osaka University Medical School
  • Ding Yun
    Department of Pharmacology, Osaka University Medical School
  • Niu San-Yong
    Department of Pharmacology, Osaka University Medical School
  • Do Eunju
    Department of Pharmacology, Osaka University Medical School
  • Miki Naomasa
    Department of Pharmacology, Osaka University Medical School

Bibliographic Information

Other Title
  • Single Stranded DNA-Binding Protein, ssCRE-BP/Purα、in Rat Lung and Its Increase in Allergic Airway Inflammation
  • Single Stranded DNA-Binding Protein ssC
  • A Single Stranded DNA-Binding Protein, ssCRE-BP/Purα, in Rat Lung and Its Increase in Allergic Airway Inflammation

Search this article

Abstract

ssCRE-BP/Purα is a single stranded DNA-binding protein and may be involved in gene replication and transcription and in the development of morphine dependence. We found a ssCRE-BP/Purα (45 kDa) in rat lung that was larger than those (40 kDa) identified in rat and mouse brains and mouse lung. Immunohistochemistry showed that ssCRE-BP/Purα is primarily distributed in the lung epithelium. As allergic inflammation induces various gene expressions, we investigated the changes of Purα during airway inflammation. Ovalbumin-sensitized rats were used for inducing allergic airway inflammation. The expression and DNA-binding activity of 45-kDa ssCRE-BP/Purα were significantly increased in the sensitized rat lungs 24 hr after antigen challenge, but not in those of rats nonsensitized or sensitized with ovalbumin and challenged with saline. Immunohistochemistry and in situ hybridization demonstrated that the vascular endothelial cells and numerous infiltrated eosinophils around the airways were stained with anti-Purα antibody. These data suggest that rat lung and the eosinophils contain a 45-kDa ssCRE-BP/Purα that is increased when airway inflammation occurs.

Journal

References(39)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top