Subspecies-Specific Targeting Mechanism of Protein Kinase C.
-
- Shirai Yasuhito
- Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
-
- Sakai Norio
- Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
-
- Saito Naoaki
- Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
書誌事項
- タイトル別名
-
- Subspecies-Specific Targeting Mechanism
この論文をさがす
抄録
To clarify the subspecies-specific functions of protein kinase C (PKC), we constructed cDNAs encoding γ-, ε- and δ-PKC fused with green fluorescent protein (GFP). All fusion proteins had enzymological and immunological characteristics similar to those of native PKCs. When expressed in CHO-K1 cells, each fusion protein showed a specific subcellular localization. Their translocations induced by various stimulation were also diverse. For example, ATP translocated γ-, ε- and δ-PKC-GFP in the cytoplasm to the plasma membrane within 30 sec with a return to the cytoplasm in 3 min, whereas TPA induced slow and irreversible translocation of all subspecies to the plasma membrane. Fatty acids also induced the translocation of γ- and ε-PKC-GFP, but the two PKC subspecies showed distinct translocation and sensitivity to various fatty acids. Furthermore, we revealed that the PKC translocation requires neither the kinase activity of PKC nor its association with cytoskeletal proteins such as F-actin. These results indicate that each subspecies has a spatially and temporally different targeting mechanism that depends on the extracellular and intracellular signals, contributing to the subspecies-specific functions of PKC. These remarkable findings also indicate that a system for monitoring the PKC translocation is a powerful tool for investigating the subspecies-specific functions of PKCs and mechanism of its translocation.
収録刊行物
-
- Jpn.J.Pharmacol.
-
Jpn.J.Pharmacol. 78 (4), 411-417, 1998
公益社団法人 日本薬理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679263818752
-
- NII論文ID
- 10008681590
-
- NII書誌ID
- AA00691188
-
- COI
- 1:CAS:528:DyaK1MXhvFWmsQ%3D%3D
-
- ISSN
- 13473506
- 00215198
-
- NDL書誌ID
- 4629545
-
- PubMed
- 9920197
-
- Web Site
- http://id.ndl.go.jp/bib/4629545
- https://ndlsearch.ndl.go.jp/books/R000000004-I4629545
- https://api.elsevier.com/content/article/PII:S0021519819310042?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819310042?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/78/4/78_4_411/_pdf
- https://search.jamas.or.jp/link/ui/1999112155
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可