Inhibition by Naloxone of Promoter Activity of the Neurofilament Gene in SK-N-SH Cells.
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- Niu San-Yong
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Kuo Che-Hui
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Taira Eiichi
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Muraoka Osamu
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Irie Yasuyuki
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Gan Ye-Hua
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Do Eunju
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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- Miki Naomasa
- Department of Pharmacology,Osaka University School of Medicine,A-6,2-2 Yamadaoka,Suita,Osaka 565-0871,Japan
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抄録
Chronic administration of morphine is known to decrease the levels of neurofilaments(NFs)in the ventral tegmental area.We ligated a promoter region of the mouse 68−KDa neurofilament(NF−68)gene to the pGL3−enhancer vector containing a luciferase gene, transfected it into SK−N−SH cells and then analyzed transcriptional activity in the cells treated with agonists or antagonists of opiate receptors.The activity of the NF−68 promoter was suppressed by naloxone about 55% at 10-5 M and 30% at 10-7 M at 48h, but suppressed not by morphine.Naltrexone at 10-5 M suppressed the promoter activity about 20%, but levallorphan, DAMGO, DPDPE and U50488 did not.The inhibition by naloxone was dose−dependent and not reversed by morphine.The inhibitory effect of naloxone was not observed in N18TG−2 cells and PC12 cells.Experiments with various deletion mutants revealed that a region responsible for naloxone suppression spans from −328 to − 101 in the gene.These results suggest that naloxone has the ability to suppress transcriptional activity in some neurons.
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 82 (1), 34-39, 2000
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282679263992704
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- NII論文ID
- 10008182601
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- NII書誌ID
- AA00691188
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- COI
- 1:CAS:528:DC%2BD3cXotF2ktg%3D%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 4966438
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- PubMed
- 10874586
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- Web Site
- http://id.ndl.go.jp/bib/4966438
- https://ndlsearch.ndl.go.jp/books/R000000004-I4966438
- https://api.elsevier.com/content/article/PII:S0021519819307255?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819307255?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/82/1/82_1_34/_pdf
- https://search.jamas.or.jp/link/ui/2000141953
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- 本文言語コード
- en
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- JaLC
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