Intracellular Ca〔2+〕 Increase in Neuro-2A Cells and Rat Astrocytes Following Stimulation of Bradykinin B2 Receptor

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  • Ikeda Yuri
    Department of Pharmacology,School of Pharmaceutical Sciences,Kitasato University,5-9-1 Shirokane,Minato-ku,Tokyo 108-8641,Japan
  • Ueno Akinori
    Department of Pharmacology,School of Pharmaceutical Sciences,Kitasato University,5-9-1 Shirokane,Minato-ku,Tokyo 108-8641,Japan
  • Naraba Hiroaki
    Department of Pharmacology,School of Pharmaceutical Sciences,Kitasato University,5-9-1 Shirokane,Minato-ku,Tokyo 108-8641,Japan
  • Matsuki Norio
    Department of Pharmacology,Faculty of Pharmaceutical Sciences,Tokyo University,7-3-1 Hongo,Bunkyo-ku,Tokyo 113-0033,Japan
  • Oh-ishi Sachiko
    Department of Pharmacology,School of Pharmaceutical Sciences,Kitasato University,5-9-1 Shirokane,Minato-ku,Tokyo 108-8641,Japan

書誌事項

タイトル別名
  • Intracellular Ca2+ Increase in Neuro-2A Cells and Rat Astrocyies Following Stimulation of Bradykinin B2 Receptor.
  • Intracellular Ca 2 Increase in Neuro 2A Cells and Rat Astrocytes Following Stimulation of Bradykinin B2 Receptor

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Murine neuroblastoma cell line Neuro−2A cells and rat brain astrocytes showed a dose−dependent increase in intracellular Ca2+ in response to bradykinin, when assessed by a single cell image analyzing system.The Ca2+ increase in Neuro−2A cells by bradykinin was also examined by a suspension fluorescent assay using fura−2 loading.The Ca2+ increase in both cases was suppressed by a bradykinin B2 receptor antagonist, Hoe 140, but not by a B1 receptor antagonist, des−Arg−Hoe 140, suggesting that the effect occurred via specific B2 receptor activation.RT−PCR for bradykinin B2 receptor mRNA showed that both Neuro−2A cells and the astrocytes expressed B2 receptor mRNA.Binding of [3H]bradykinin to Neuro−2A cells was assessed, and a specific binding constant of 0.75 nM was determined.Furthermore, the increase in [Ca2+]i by bradykinin could be caused by a release of Ca2+ from storage sites in the endoplasmic reticulum, since thapsigargin and U−73122 attenuated the effect of bradykinin in Neuro−2A as well as in astrocytes.These results indicate that both astrocytes and neuroblastoma Neuro−2A cells stimulated by bradykinin could express a bradykinin B2 receptor−mediated intracellular Ca2+ increase leading to signal transduction.

収録刊行物

  • Jpn.J.Pharmacol.

    Jpn.J.Pharmacol. 84 (2), 140-145, 2000

    公益社団法人 日本薬理学会

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