RABBIT LIVER β-GLUCURONIDASE

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  • OHISHI Iwao
    Biochemical Pharmacology Laboratory, Research Laboratories, Chugai Pharmaceutical Co., Ltd.
  • SHIOYA Akitoshi
    Biochemical Pharmacology Laboratory, Research Laboratories, Chugai Pharmaceutical Co., Ltd.

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  • Purification and its properties
  • I. PURIFICATION AND ITS PROPERTIES

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Abstract

It has previously been shown from our study using the rabbit as an experimental animal that the increase in serum β-glucuronidase activity is positively related to insulin-dependent-change in blood glucose levels (1-2). While this finding may be associated with the observed elevation of serum β-glucuronidase activity in the patient with diabetes mellitus (3-5) and in the diabetic rabbit following administration of alloxan (2), the origin of this activity has not been identified completely. A suggestion can only be made that the liver is responsible for the increased serum enzyme activity, on the basis of the decreased β-glucuronidase activity in this organ after administration of glucose (1). To determine the origin of the activity more precisely, it was decided to employ an immunological method by using rat anti-serum to highly purified rabbit liver β-glucuronidase.<BR> This paper thus describes purification and characterization of liver β-glucuronidase of rabbit which is suitable for further immunochemical study.

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