Butanol extracts from myelin fragments-2-Some properties of 5-hydroxytryptamine binding

DOI Web Site Web Site Web Site PubMed 被引用文献1件 参考文献34件
  • ISHITANI Ryoichi
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
  • MIYAKAWA Akihisa
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
  • IWAMOTO Takio
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University

書誌事項

タイトル別名
  • Butanol extracts from myelin fragments-II. Some properties of 5-hydroxytryptamine binding.
  • Butanol extracts from myelin fragments
  • Butanol extracts from myelin fragments-. Some properties of 5-hydroxytryptamine binding

この論文をさがす

抄録

The myelin fragments of rat brain stem were treated with butanol-water mixtures, and the butanol extracts (total extract=TE) were incubated with 5×10-7 M of C14•5-HT. After incubation, protein, lipid phosphorus and radioactivity were analyzed by Sephadex LH20 column chromatography. Two peaks of components eluted in chloroform-methanol 4:1 (peak I and II) showed the binding capacity for C14•5-HT. The displacement studies with unlabeled 5-HT (5×10-4 M) suggested that peak II was the saturable binding component to 5-HT. On the other hand, butanol extracts from the synaptic membranes of rat brain stem did not show binding for C14•5-HT. Various compounds were studied to determine their inhibitory effects on the saturable binding of 5-HT to TE. The results indicated that acetylcholine, dopamine and tryptamine inhibited the 5-HT binding but LSD, reserpine, colchicine, vinblastine, 5-hydroxytryptophan (5-HTP) and 5-hydroxy-3-indole acetic acid (5-HIAA) had no effect.

収録刊行物

  • Jpn.J.Pharmacol.

    Jpn.J.Pharmacol. 28 (6), 899-907, 1978

    公益社団法人 日本薬理学会

被引用文献 (1)*注記

もっと見る

参考文献 (34)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ